"Oxidative stress in patients with multiple sclerosis"
Syburra C; Passi S, Ukr Biokhim Zh 1999 May-Jun;71(3):112-5
ABSTRACT: It is well known that brain and nervous system cells are prone
to oxidative damage because of their relatively low content of antioxidants,
especially enzymatic ones, and of the high levels of both membrane polyunsaturated
fatty acids (PUFA) and iron easily released from injured cells. We have
investigated the oxidative stress in the blood (plasma, erythrocytes and
lymphocytes) of 28 patients affected with multiple sclerosis (MS) and
of 30 healthy age matched controls, by performing a multiparameter analysis
of non-enzymatic and enzymatic antioxidants--Vitamin E (Vit. E), ubiquinone
(UBI), reduced and oxidized glutathione (GSH, GS-SG), superoxide dismutase
(SOD), glutathione peroxidase (GPX), catalase (CAT) and fatty acid patterns
of phospholipids (PL-FA). PL-FA and Vit. E were assayed by GC-MS; UBI
and GSH/GS-SG by HPLC; SOD, GPX and CAT by spectrophotometry. In comparison
to controls, patients with MS showed significantly reduced levels of plasma
UBI (0.21 +/- 0.10 vs. 0.78 +/- 0.08 mg/ml, p < 0.001), plasma Vit.
E (7.4 +/- 2.1 vs. 11.4 +/- 1.8 mg/ml, p < 0.01), lymphocyte UBI (8.1
+/- 4.0 vs. 30.3 +/- 7.2 ng/ml blood, p < 0.001) and erythrocyte GPX
(22.6 +/- 5.7 vs. 36.3 +/- 6.4 U/g Hb, p < 0.001). This blood antioxidant
deficiency was associated with plasma levels of PL-PUFA--especially C20:3
n-6 and C20:4 n-6--significantly higher than controls.
In conclusion, the blood of patients with MS shows the signs of a significant
oxidative stress. The possibility of counteracting it by antioxidant administration
plus an appropriate diet, might represent a promising way of inhibiting
the progression of the disease. Antioxidant supplements should include
not only GSH repleting agents, but also Vit. E, ubiquinol, and selenium.